Xray crystallography has long been a vital method for studying the structureof proteins and other macromolecules. As the importance of proteins continuesto grow... » 

Xray crystallography has long been a vital method for studying the structureof proteins and other macromolecules. As the importance of proteins continuesto grow in fields from biochemistry and biophysics to pharmaceuticaldevelopment and biotechnology many researchers have found that a knowledge ofXray diffraction is an indispensable tool. In this new edition of hisessential work Dr. Jan Drenth recognized internationally for his numerouscontributions to crystallographic research has provided an uptodate andtechnically rigorous introduction to the subject. Principles of Protein XrayCrystallography provides the theoretical background necessary to understand howthe structure of proteins is determined at atomic resolution. It is intended toserve as an introduction for graduate students postdoctoral researchers andestablished scientists who want to use protein crystallography in their ownendeavors or need to understand the subject in order to critically evaluatethe literature. New additions to the book include a section on twinning anadditional chapter on crystal growth and a discussion of singlewavelengthanomalous dispersion SAD. TOCPrefaces. Crystallizing a Protein. XRaySources and Detectors. Crystals. The Theory of XRay Diffraction by aCrystal. Average Reflection Intensity and Distribution of Structure FactorData. Special Forms of the Structure Factor. The Solution of the PhaseProblem by the Isomorphous Replacement Method. Phase Improvement. AnomalousScattering in the Determination of the Protein Phase Angles and the AbsoluteConfiguration. Molecular Replacement. Direct Methods. Laue Diffraction.Refinement of the Model Structure. The Combination of Phase Information.Checking for Gross Errors and Estimating the Accuracy of the Structural Model.Tips and Tricks in Protein Crystallization. Appendices. References. Index. «