The logic of the overall approach to protein isolation is explained and thephysical principles of each separation method are made clear by the use ofsimple mode... » 

The logic of the overall approach to protein isolation is explained and thephysical principles of each separation method are made clear by the use ofsimple models and analogies drawn from everyday experiences. The authors aimhas been to deepen the readers insight into protein isolation methods so thatthey may tackle new problems and perhaps devise new approaches to old problems.Many of the methods described are drawn from the authors own research and arethus uniquely described here examples are threephase partitioning nonlinearelectrophoresis and a simple approach to buffer making. In this 2nd editionthe treatment of the basic physical principles has been expanded and clarifiedthe importance of ionic strength in measuring enzyme pH optima is emphasisedand a computer program for the calculation of buffers of defined ionic strengthis provided. The section on threephase partitioning has been expanded toinclude the latest research findings on the use of tbutanol to inhibit enzymesand minimise homogenisation artefacts the treatment of HPLC has been expandedand the most common practical methods are explained in detail in a new chapter.Additional study questions are provided as are the answers to all studyquestions. «